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Three methods were applied to identify the EPCs 27 : morphology of EPCs, double staining with 1,1-dioctadecyl-3,3,3,3-tetramethylindocarbocyanine perchlorate-labeled acetylated low-density lipoprotein (Dil-acLDL from Eugene, USA) and fluorescein isothiocyanate-labeled Ulex europaeus agglutinin-1 (FITC-UEA-1 from Sigma, USA), and co-expressing of FITC-CD34 + / PE-CD133 + / APC-Flk-1 + (FITC-CD34 and APC-Flk-1 from Becton Dickinson, USA